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Your present location:HomeProductsRestriction Enzyme and Modification EnzymeFast Restriction Enzyme / FlyCut® NotI
  • Product Name: FlyCut® NotI
  • Catalog Number: JN401
  • Date: 2016-06-20
  • Views: 46
  • Sold amount:0Piece
    • Specification: 250 units 500 units
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Overview

Description

FlyCut® NotI is expressed and purified from E.coli that carries the recombinant NotI gene. The molecular weight is 43.3 kDa, with the recognition site at GC^GGCCGC. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Concentration 10,000 units/ml

Recognition Site

5’...GC↓GGCCGC...3’

3’...CGCCGG↑CG...5’

Applications

Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

Citations & references

 

 

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