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Your present location:HomeProductsRestriction Enzyme / FlyCut® SacI
  • Product Name: FlyCut® SacI
  • Catalog Number: JS101
  • Date: 2016-06-20
  • Views: 18
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Overview

Description

FlyCut® SacI is expressed and purified from E.coli that carries the recombinant SacI gene. The molecular weight is 40.8 kDa, with the recognition site at GAGCT^C. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Concentration 20,000 units/ml

Recognition Site

5’...GAGCT↓C...3’

3’...C↑TCGAG...5’

Applications

Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

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