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Your present location:HomeProductsRestriction Enzyme / FlyCut SmaI
  • Product Name: FlyCut SmaI
  • Catalog Number: JS501
  • Date: 2016-06-20
  • Views: 4
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Overview

Description

.FlyCutTM SmaI is expressed and purified from E.coli that carries the recombinant SmaI gene. The molecular weight is 29.7 kDa, with the recognition site at CCC^GGG The reaction is conducted for 5-10 minutes at 25oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.

Concentration 20,000 units/ml

Recognition Site

5’...CCC↓GGG...3’

3’...GGG↑CCC...5’

Applications

Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

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