transbionovo

+86-10-57815027      
中文版 | ENGLISH

Your present location:HomeProductsRestriction Enzyme / FlyCut SpeI
  • Product Name: FlyCut SpeI
  • Catalog Number: JS601
  • Date: 2016-06-20
  • Views: 3
  • Sold amount:0Piece
    • Specification: 250 units 500 units
  • Quantity:  

Favorite

 

Overview Contents& storage Citations & references Related Images Download  
Overview

Description

.FlyCutTM SpeI is expressed and purified from E.coli that carries the recombinant SpeI gene. The molecular weight is 21.7 kDa, with the recognition site at A^CTAGT. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Concentration 10,000 units/ml

Recognition Site

5’...A↓CTAGT...3’

3’...TGATC↑A...5’

Applications

Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

Citations & references

 

 

Related Images

  To be closed automatically in X seconds

View my favorites