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  • Product Name: FlyCut® XmaI
  • Catalog Number: JX301
  • Date: 2016-06-20
  • Views: 93
  • Sold amount:0Piece
    • Specification: 250 units 500 units
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Overview

Description

FlyCut® XmaI is expressed and purified from E.coli that carries the recombinant XmaI gene. The molecular weight is 37.6 kDa, with the recognition site at C^CCGGG. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Concentration 10,000 units/ml

Recognition Site

5’...C↓CCGGG...3’

3’...GGGCC↑C...5’

Applications

Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
The spatial distribution of nitrogen removal functional genes in multimedia biofilters for sewage treatment Ecological Engineering 2.958 Ji G, et al. 2013 Jun http://www.sciencedirect.com/science/article/pii/S0925857413000876
Molecular Cloning, Characterization, and mRNA Expression of Two Cryptochrome Genes in Helicoverpa armigera (Lepidoptera: Noctuidae) Journal of Economic Entomology 1.699 Yan S, et al. 2013 Feb http://www.ncbi.nlm.nih.gov/pubmed/23448062
The Complete Mitochondrial Genome of Bean Goose (Anser fabalis) and Implications for Anseriformes Taxonomy PloS one 3.73 Gang Liu et,al.    2013 May http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0063334
The role of aromatic L-amino acid decarboxylase in bacillamide C biosynthesis by Bacillus atrophaeus C89 Scientific reports 5.578 L Yuwen et,al.  2013 Apr http://www.nature.com/srep/2013/130430/srep01753/full/srep01753.html?WT.ec_id=SREP-20130507#affil-auth
Population Genetic Structure of the Invasive Red Swamp Crayfish in China Revealed by ITS1 Variation Biochemical genetics 0.865 Gang Liu et,al.  2013 Dec http://link.springer.com/article/10.1007/s10528-013-9611-z#
Association of nitrogen micro-cycle functional genes in subsurface wastewater infiltration systems Ecological Engineering 2.958 Ji G, et al. 2012 Jul http://www.sciencedirect.com/science/article/pii/S0925857412001255
Diversity and evolution of the Wolbachia endosymbionts of Bemisia (Hemiptera: Aleyrodidae) whiteflies Ecology and Evolution 2.32 XL Bing, et al.    2014 Jun http://onlinelibrary.wiley.com/doi/10.1002/ece3.1126/full
Distribution patterns of denitrification functional genes and microbial floras in multimedia constructed wetlands Ecological Engineering 2.958 Ji G, et al. 2012 Jul http://www.sciencedirect.com/science/article/pii/S0925857412001103

 

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