pEASY®-Blunt E2 Expression Vector is constructed from pET vector. It utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.
• 5 minutes fast ligation of Pfu-amplified PCR products.
• Ampicillin resistance.
• T7 promoter primer and T7 terminator primer for sequencing.
• Bacteriophage T7lac promoter for high level expression.
• C-terminal 6×His tag for easy purification.
• Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast
• E2 Expression Plasmid included as negative control.