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Your present location:HomeProductsGene ExpressionExpression Competent Cells / BL21 (DE3) pLysS Chemically Competent Cell
  • Product Name: BL21 (DE3) pLysS Chemically Competent Cell
  • Catalog Number: CD701
  • Date: 2016-06-27
  • Views: 70
  • Sold amount:0Piece
    • Specification: 10×100 µl 20×100 µl
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Overview

Description

BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to chloramphenicol(CamR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).
 

Genotype

F- ompT hsdS(rB-mB-)gal dcm(DE3)pLysS Camr
 

Features

• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• CamR.
• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without
  disturbing IPTG functioning.
• Suitable for non-toxic and toxic protein’s expression.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

 

Contents& storage

 

Citations & references
Literature Journal IF Author Date Link
Improved production of holotoxin Stx2 with biological activities by using a single-promoter vector and an auto-induction expression system. Protein Expression and Purification 1.429 Tu W, et al. 2009 Oct http://www.ncbi.nlm.nih.gov/pubmed/?term=19450690
A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell World Journal of Microbiology & Biotechnology 1.262 X-T. Yin, et al. 2010 Aug http://link.springer.com/article/10.1007/s11274-010-0308-8
Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxin Biosensors and Bioelectronics 5.437 Chang Zhang, et al.    2014 Apr http://www.sciencedirect.com/science/article/pii/S0956566313007409
A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11 J Virol.  4.439 Yuejun Shi, et al.       2016 Feb http://www.ncbi.nlm.nih.gov/pubmed/?term=A+Dimerization-Dependent+Mechanism+Drives+the+Endoribonuclease+Function+of+PRRSV+nsp11
Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor Appl Microbiol Biotechnol 3.689 Chang Zhang, et al. 2013 Jul http://link.springer.com/article/10.1007/s00253-013-5121-5
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