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  • Product Name: BL21 (DE3) pLysS Chemically Competent Cell
  • Catalog Number: CD701
  • Date: 2016-06-27
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    • Specification: 10×100 µl 20×100 µl
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BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to chloramphenicol(CamR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).


F- ompT hsdS(rB-mB-)gal dcm(DE3)pLysS Camr


• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• CamR.
• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without
  disturbing IPTG functioning.
• Suitable for non-toxic and toxic protein’s expression.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.


Contents& storage


Citations & references
Literature Journal IF Author Date Link
Improved production of holotoxin Stx2 with biological activities by using a single-promoter vector and an auto-induction expression system. Protein Expression and Purification 1.429 Tu W, et al. 2009 Oct
A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell World Journal of Microbiology & Biotechnology 1.262 X-T. Yin, et al. 2010 Aug
Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxin Biosensors and Bioelectronics 5.437 Chang Zhang, et al.    2014 Apr
A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11 J Virol.  4.439 Yuejun Shi, et al.       2016 Feb
Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor Appl Microbiol Biotechnol 3.689 Chang Zhang, et al. 2013 Jul
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