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  • Product Name: TransB(DE3) Chemically Competent Cell
  • Catalog Number: CD811
  • Date: 2016-06-27
  • Views: 35
  • Sold amount:0Piece
    • Specification: 10×100 µl
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Overview

Description

BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).
 

Genotype

F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR,TetR)
 

Features

• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• KanR and TetR.
• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

 

Contents& storage

 

Citations & references

 

Literature Journal IF Author Date Link
Biochemical characteristics of New Delhi metallo-β-lactamase-1 show unexpected difference to other MBLs. PLoS One 3.73 T Li, et al. 2013 Apr http://www.ncbi.nlm.nih.gov/pubmed/23593503
Expression and Characterization of a Recombinant Laccase with Alkalistable and Thermostable Properties from Streptomyces griseorubens JSD-1 Applied Biochemistry and Biotechnology 1.735 Haiwei Feng, et al.      2015 Mar http://link.springer.com/article/10.1007/s12010-015-1594-2

 

 

 

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