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  • Product Name: Transetta(DE3) Chemically Competent Cell
  • Catalog Number: CD801
  • Date: 2016-06-27
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Overview

Description

Transetta(DE3) Chemically Competent Cell is specifically designed from imported strain for chemical transformation of DNA. It is resistant to chloramphenicol(CamR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).
 

Genotype

F- ompT hsdSB(rB- mB-) gal dcm lacY1(DE3)pRARE(argU, argW, ileX, glyT, leuW, proL)(Camr)
 

Features

• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• CamR.
• tRNAs for 6 rare codons AUA, AGG, AGA, CUA, CCC, GGA. Enhance the expression level of proteins in the prokaryotic system.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

 

Contents& storage

 

Citations & references

 

Literature Journal IF Author Date Link
Exploring the Catalytic Promiscuity of a New Glycosyltransferase from Carthamus tinctorius
Organic Letters 6.2 Kebo Xie, et al      2014 Sep http://pubs.acs.org/doi/abs/10.1021/ol502380p
Mesomartoxin, a new Kv1.2-selective scorpion toxin interacting with the channel selectivity filter Biochemical Pharmacology 5.009 Xueli Wang, et al.        2014 Dec http://www.sciencedirect.com/science/article/pii/S0006295214006972
A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense PLoS One 3.73 Q Jiang, et al. 2013 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0069158
Systematic functional comparative analysis of four single-stranded DNA-binding proteins and their affection on viral RNA metabolism. PLoS One 3.73 H Shi, et al. 2013 Jan http://www.ncbi.nlm.nih.gov/pubmed/23365690

 

 

 

 

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