TransDetect® Double-Luciferase Reporter Assay Kit provides an efficient method to perform dual-reporter assays. In the assay, the
activities of firefly (Photinus pyralis) and Renilla (sea pansy) luciferases are measured sequentially from a single sample. The
firefly luciferase reporter is measured firstly by adding Luciferase Reaction Reagent to generate a luminescent signal. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated by adding Luciferase Reaction Reagent II to the same tube.