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Your present location:HomeBest SellersEasyTaq® DNA Polymerase / EasyTaq® DNA Polymerase
  • Product Name: EasyTaq® DNA Polymerase
  • Catalog Number: AP111
  • Date: 2016-09-05
  • Views: 59
  • Sold amount:0Piece
    • Specification: 500 units 6×500 units 10×5000 units 4×2500 units 500 Units 6×500 Units 4×2500 Units
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Overview Contents& storage Citations & references Related Images Download  
Overview

Description

EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.

 

Highlights

• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

 

Applications

• Routine PCR
• Colony PCR

 

Unit Definition

One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

EasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

 

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

 

10×EasyTaq® Buffer (with Mg2+)

200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Lack of Structural Variation but Extensive Length Polymorphisms and Heteroplasmic Length Variations in the Mitochondrial DNA Control Region of Highly Inbred Crested Ibis, Nipponia nippon PLoS One 3.73 He XL, et al. 2013 Jun http://www.ncbi.nlm.nih.gov/pubmed/23805212
MACRO: A Combined Microchip-PCR and Microarray System for High-throughput Monitoring of Genetically Modified Organisms ACS 12.113 Ning Shao,et al.   2013 Dec http://pubs.acs.org/doi/abs/10.1021/ac403630a
Evolutionary relationships of Pemphigus and allied genera (Hemiptera: Aphididae: Eriosomatinae) and their primary endosymbiont, Buchnera aphidicola Insect Science 2.144 L Liu et al.      2014 Jan http://onlinelibrary.wiley.com/doi/10.1111/1744-7917.12113/abstract
Detection of Roundup Ready soybean by loop-mediated isothermal amplification combined with a lateral-flow dipstick Food Control 2.806 Xiumin Wang et al.      2013 Jan http://www.sciencedirect.com/science/article/pii/S0956713512003271
Molecular diversity of class 2 integrons in antibiotic-resistant gram-negative bacteria found in wastewater environments in China Ecotoxicology 2.706 Ruirui Xia et al. 2013 Mar http://link.springer.com/article/10.1007/s10646-012-1034-9#
Hydrogen Sulfide Alleviates Postharvest Senescence of Broccoli by Modulating Antioxidant Defense and Senescence-Related Gene Expression J AGR FOOD CHEM  2.912 Shi-Ping Li et al 2014 Jan http://pubs.acs.org/doi/abs/10.1021/jf4047122
Identification of Plasmid-Mediated Quinolone Resistance qnr Genes in Multidrug-Resistant Gram-Negative Bacteria from Hospital Wastewaters and Receiving Waters in the Jinan Area, China Microbial Drug Resistance 2.49 Ruirui Xia et al.  2013 Jul http://online.liebertpub.com/doi/abs/10.1089/mdr.2012.0210
Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers Chinese Journal of Oceanology and Limnology 0.657 Shenhui Yu et al.   2013 May http://link.springer.com/article/10.1007/s00343-013-2064-8#
Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong Journal of Applied Microbiology 2.479 W.sun et,el.   2013 Nov http://onlinelibrary.wiley.com/doi/10.1111/jam.12367/abstract?deniedAccessCustomised
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Rapid Diversification of FoxP2 in Teleosts through Gene Duplication in the Teleost-Specific Whole Genome Duplication Event PloS one 3.73 Xiaowei Song et al.      2013 Dec http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0083858
Generic status, circumscription, and allopolyploid origin of Faberia (Asteraceae: Cichorieae) as revealed by ITS and chloroplast DNA sequence data Taxon 3.299 Liu, Ying, et al.     2013 Dec

http://www.ingentaconnect.com/content/iapt/tax/2013/00000062/00000006/art00010

Resistance to the Novel Fungicide Pyrimorph in Phytophthora capsici: risk assessment and detection of point mutations in CesA3 that confer resistance PLoS One 3.73 Pang Z, et al. 2013 http://www.ncbi.nlm.nih.gov/pubmed/23431382
Transcriptome-wide identification and characterization of microRNAs from castor bean (Ricinus communis L.) PLoS One 3.73 Xu W, et al. 2013 Jul  http://www.ncbi.nlm.nih.gov/pubmed/23894571
Molecular diversity of arbuscular mycorrhizal fungi associated with two cooccurring perennial plant species on a Tibetan altitudinal gradient Mycorrhiza 2.955 Li X, et al. 2013 Aug  http://www.ncbi.nlm.nih.gov/pubmed/23912811
Polymorphisms of anti-lipopolysaccharide factors in the swimming crab Portunus trituberculatus and their association with resistance/susceptibility to Vibrio alginolyticus Fish & Shellfish Immunology 2.964 Li X, et al.  2013 Jun http://www.ncbi.nlm.nih.gov/pubmed/23567857
Nonstructural proteins of Torque teno sus virus 2 from O2AUG: Preduction to experimental validation Virus Research 2.745 Lu Y, et al. 2013 Sep  http://www.ncbi.nlm.nih.gov/pubmed/24091363
Highly parallel single-molecule amplification approach based on agarose droplet polymerase chain reaction for efficient and cost-effective aptamer selection Analytical Chemistry 5.695 Zhang WY.  2012 Jan  http://www.ncbi.nlm.nih.gov/pubmed/22103644
 In vitro assembly of multiple DNA fragments using successive hybridization PLoS One 3.73 Jiang X, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/22291927
In vitro selection of highly efficient G-quadruplex-based DNAzymes Analytical Chemistry 5.695 Zhu L, et al. 2012 Oct  http://www.ncbi.nlm.nih.gov/pubmed/22954361
Extracting genomic DNA of foodstuff by polyamidoamine (PAMAM)-magnetite nanoparticles Talanta 3.498 Qie F, et al. 2012 May  http://www.ncbi.nlm.nih.gov/pubmed/22483894
Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs Evidence-based Complementary and Alternative Medicine: eCAM 4.774 Wu F, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/23346217
Assessing the risk that Phytophthora melonis can develop a point mutation (V1109L) in CesA3 conferring resistance to carboxylic acid amide fungicides PLoS One 3.73 Chen L, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/22848705
Development of a simvastatin selection marker for a hyperthermophilic acidophile, Sulfolobus islandicus Applied and Environmental Microbiology 3.829 Zheng T, et al. 2012 Jan http://www.ncbi.nlm.nih.gov/pubmed/22081574
Elastolytic mechanism of a novel M23 metalloprotease pseudoalterin from deep-sea Pseudoalteromonas sp. CF6-2: cleaving not only glycyl bonds in the hydrophobic regions but also peptide bonds in the hydrophilic regions involved in cross-linking The Journal of Biological Chemistry 4.773 Zhao HL, et al. 2012 Nov  http://www.ncbi.nlm.nih.gov/pubmed/23012370 
Molecular cloning and characterization of an endogenous digestive β-glucosidase from the midgut of the fungus-growing termite Macrotermes barneyi Insect Molecular Biology 2.529 Wu Y, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/23126269
Identification of novel variants of metadherin in breast cancer PLoS One 3.73 Liu X. et al.  2011 Mar  http://www.ncbi.nlm.nih.gov/pubmed/21408129 
A universal multiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray Lab On A Chip 5.67 Li Y. et al. 2011 Nov  http://www.ncbi.nlm.nih.gov/pubmed/21909519
Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv International Journal of Molecular Sciences 2.598 Wang DW. et al. 2011 Jan  http://www.ncbi.nlm.nih.gov/pubmed/21340018
BCL-2 (-938C > A) polymorphism is associated with breast cancer susceptibility BMC Medical Genetics 2.328 Zhang N. et al. 2011 Apr  http://www.ncbi.nlm.nih.gov/pubmed/21457555
Angiostrongylus vasorum from South America and Europe represent distinct lineages Parasitology 2.961 Jefferies R. et al. 2009 Jan http://www.ncbi.nlm.nih.gov/pubmed/19126274
Expression and evolutionary divergence of the non-conventional olfactory receptor in four species of fig wasp associated with one species of fig BMC Evolutionary Biology 3.521 Lu B. et al.  2009 Feb  http://www.ncbi.nlm.nih.gov/pubmed/19232102
Frequency of virulence factors in Escherichia coli isolated from suckling pigs with diarrhoea in China The Veterinary Journal 2.424 Wenxin Liu, et al. 2014 Feb http://www.sciencedirect.com/science/article/pii/S1090023313006114
Two Non-target Recessive Genes Confer Resistance to the Anti-Oomycete Microtubule Inhibitor Zoxamide in Phytophthora capsici PloS one 3.73 Yang Bi, et al. 2014 Feb http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0089336
BIOTYPE EXPRESSION AND INSECTICIDE RESPONSE OF Bemisia tabaci CHEMOSENSORY PROTEIN-1 Insect Biochemistry and Physiology 1.515 Guoxia Liu, et al. 2014 Mar http://onlinelibrary.wiley.com/doi/10.1002/arch.21148/abstract?deniedAccessCustomised
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MACRO: A Combined Microchip-PCR and Microarray System for High-Throughput Monitoring of Genetically Modified Organisms Analytical chemistry 5.695 Ning Shao, et al. 2014 http://pubs.acs.org/doi/abs/10.1021/ac403630a
The autophagy-related gene Acatg1 is involved in conidiation and cephalosporin production in Acremonium chrysogenum Fungal Genetics and Biology 3.263 Haoting Wang, et al. 2014 Aug http://www.sciencedirect.com/science/article/pii/S1087184514001078
TTAGG‐repeat telomeres and characterization of telomerase in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae) Insect Molecular Biology 2.589 H. Gong, et al.   2015 Feb http://onlinelibrary.wiley.com/doi/10.1111/imb.12163/abstract;jsessionid=
B467C4FAFC6CAF771B78035F28965698.f04t01?deniedAccessCustomised
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Molecular Mechanism of Mesosulfuron-methyl Resistance in Multiple-resistant American Sloughgrass (Beckmannia syzigachne Steud.) Weed Science 1.87 Lingxu Li, et al.    2015 Feb http://wssajournals.org/doi/abs/10.1614/WS-D-15-00026.1
Development of a high-efficiency gene knockout system for Pochonia chlamydosporia Microbiological Research 2.561 Baoming Shen, et al.   2015 Jan http://www.sciencedirect.com/science/article/pii/S0944501314001141
Regulation of myo -inositol catabolism by a GntR-type repressor SCO6974 in Streptomyces coelicolor Applied Microbiology and Biotechnology 3.337 Lingjun Yu, et al.    2015 Apr http://link.springer.com/article/10.1007/s00253-014-6368-1
Ancient association with Fagaceae in the aphid tribe Greenideini (Hemiptera: Aphididae: Greenideinae) Systematic Entomology 2.784 QING-HUA LIU, et al.   2014 Sep http://onlinelibrary.wiley.com/doi/10.1111/syen.12100/pdf
Polymorphisms and DNA methylation level in the CpG site of the GHR1 gene associated with mRNA expression, growth traits and hormone level of half-smooth tongue sole (Cynoglossus semilaevis ) Fish Physiology and Biochemistry 1.622 J. L. Zhao, et al.    2015 Apr http://link.springer.com/article/10.1007/s10695-015-0052-y
SNP Affects the Mobility of Breast Cancer Cells and the Expression of Metastasis-Related Genes Advances in Applied Biotechnology 2.737 Juan Hu, et al.  2015 Apr http://link.springer.com/chapter/10.1007/978-3-662-46318-5_20
Characterization of fourteen single nucleotide polymorphism markers in the Manila clam (Ruditapes philippinarum ) Conservation Genetics Resources 1.172 Hongtao Nie, et al.     2015 Mar http://link.springer.com/article/10.1007/s12686-014-0292-7
Analysis of the Transcriptome of Erigeron breviscapus Uncovers Putative Scutellarin and Chlorogenic Acids Biosynthetic Genes and Genetic Markers Plos one 3.73 Ni-Hao Jiang, et al. 2014 Jun http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0100357#pone-0100357-g009

 

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