Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRPCR Enzyme / EasyTaq® DNA Polymerase for PAGE
  • Product Name: EasyTaq® DNA Polymerase for PAGE
  • Catalog Number: AP112
  • Date: 2015-10-25
  • Views: 142
  • Sold amount:0Piece
    • Specification: 2500 units 4×2500 units 2500 Units(With dNTPs) 4×2500 Units(With dNTPs)
  • Quantity:  

Purchase Favorite


Overview Contents& storage Citations & references Related Images Download  





EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.



• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 3 kb.



Short fragment PCR


Unit Definition

One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

EasyTaq® DNA Polymerase for PAGE has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase for PAGE has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers


10×EasyTaq® Buffer for PAGE (with Mg2+)

200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others



Contents & storage



Citations & references


Literature Journal IF Author Date Link
Isolation and characterization of microsatellite loci in hybrid giant tiger grouper Genetics and Molecular Research 0.764 Huang YS, et al. 2015 Nov



Related Images

  To be closed automatically in X seconds

View my favorites