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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRPCR Enzyme / TransTaq-T DNA Polymerase
  • Product Name: TransTaq-T DNA Polymerase
  • Catalog Number: AP122
  • Date: 2015-10-25
  • Views: 42
  • Sold amount:0Piece
    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
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Overview

 

Description

TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq®DNA Polymerase. It is more suitable for high fidelity TA cloning.

 

Highlights

TransTaq®-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.

 

Applications

• Complex templates
• TA cloning

 

Unit Definition

One unit of TransTaq®-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

TransTaq®-T DNA Polymerase has passed t he following quality control assays:functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq®-T DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. 

 

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

 

10×TransTaq®-T Buffer

200 mM Tris-HCl (pH 9.0), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
The spatial distribution of nitrogen removal functional genes in multimedia biofilters for sewage treatment Ecological Engineering 2.958 Ji G, et al. 2013 Jun http://www.sciencedirect.com/science/article/pii/S0925857413000876
Molecular Cloning, Characterization, and mRNA Expression of Two Cryptochrome Genes in Helicoverpa armigera (Lepidoptera: Noctuidae) Journal of Economic Entomology 1.699 Yan S, et al. 2013 Feb http://www.ncbi.nlm.nih.gov/pubmed/23448062
The Complete Mitochondrial Genome of Bean Goose (Anser fabalis) and Implications for Anseriformes Taxonomy PloS one 3.73 Gang Liu et,al.    2013 May http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0063334
The role of aromatic L-amino acid decarboxylase in bacillamide C biosynthesis by Bacillus atrophaeus C89 Scientific reports 5.578 L Yuwen et,al.  2013 Apr http://www.nature.com/srep/2013/130430/srep01753/full/srep01753.html?WT.ec_id=SREP-20130507#affil-auth
Population Genetic Structure of the Invasive Red Swamp Crayfish in China Revealed by ITS1 Variation Biochemical genetics 0.865 Gang Liu et,al.  2013 Dec http://link.springer.com/article/10.1007/s10528-013-9611-z#
Association of nitrogen micro-cycle functional genes in subsurface wastewater infiltration systems Ecological Engineering 2.958 Ji G, et al. 2012 Jul http://www.sciencedirect.com/science/article/pii/S0925857412001255
Diversity and evolution of the Wolbachia endosymbionts of Bemisia (Hemiptera: Aleyrodidae) whiteflies Ecology and Evolution 2.32 XL Bing, et al.    2014 Jun http://onlinelibrary.wiley.com/doi/10.1002/ece3.1126/full
Distribution patterns of denitrification functional genes and microbial floras in multimedia constructed wetlands Ecological Engineering 2.958 Ji G, et al. 2012 Jul http://www.sciencedirect.com/science/article/pii/S0925857412001103

 

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