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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRPCR Enzyme / TransTaq DNA Polymerase High Fidelity(HiFi)
  • Product Name: TransTaq DNA Polymerase High Fidelity(HiFi)
  • Catalog Number: AP131
  • Date: 2015-10-25
  • Views: 36
  • Sold amount:0Piece
    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
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Overview Contents& storage Citations & references Related Images Download  

 

Overview

 

Description

TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease. TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.

 

Highlights

TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.

 

Applications

• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR

 

Unit Definition

One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in
30 minutes at 74℃.

 

Quality Control

TransTaq® HiFi DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq® HiFi DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA

 

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

 

10×TransTaq® HiFi Buffer

200 mM Tris-HCl (pH 9.0), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 10% Glycerol, others

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi PLoS One 3.73 Wang B. et al. 2013 http://www.ncbi.nlm.nih.gov/pubmed/23342036
Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica Maxim Journal of Integrative Plant Biology 2.534 Li M, et al. 2013 Jun http://www.ncbi.nlm.nih.gov/pubmed/23480422
The improved l-tryptophan production in recombinant Escherichia coli by expressing the polyhydroxybutyrate synthesis pathway Applied Microbiology and Biotechnology 3.689 Gu P, et al. 2013 May http://www.ncbi.nlm.nih.gov/pubmed/23321909
Phylogenetic analysis using E2 gene of classical swine fever virus reveals a new subgenotype in China Infection, Genetics and Evolution 3.015 DL Jiang et al.       2013 July http://www.sciencedirect.com/science/article/pii/S1567134813001433
A Modified TALEN-Based Strategy for Rapidly and Efficiently Generating Knockout Mice for Kidney Development Studies PloS one 3.73 Yunhong Liu,et al,      2014 Jan http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0084893
New Amynthas species (Oligochaeta: Megascolecidae) from south and central Hainan Island, China and estimates of evolutionary divergence among some corticis-group species Journal of Natural History  0.881 Jing Suna et,al.      2013 Feb http://www.tandfonline.com/doi/abs/10.1080/00222933.2012.743613
Modulation of calcineurin activity in mouse brain by chronic oral administration of cyclosporine A IUBMB Life 2.789 Liu H, et al. 2013 May http://www.ncbi.nlm.nih.gov/pubmed/23436429
One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli Microbial Cell Factories 3.552 Gu P. et al. 2012 Mar  http://www.ncbi.nlm.nih.gov/pubmed/22380540 
Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition Plant Physiology and Biochemistry 2.838 Xiang L. et al.  2012 Sep http://www.ncbi.nlm.nih.gov/pubmed/22796899
Kernel lysine content does not increase in some maize opaque2 mutants Planta 3 Zhao G. et al.  2012 Jan http://www.ncbi.nlm.nih.gov/pubmed/21870098
Nuclear and plastid haplotypes suggest rapid diploid and polyploid speciation in the N Hemisphere Achillea millefolium complex (Asteraceae) BMC Evolutionary Biology 3.521 Guo YP. et al.  2012 Jan  http://www.ncbi.nlm.nih.gov/pubmed/22214230
Plasmid construction for genetic modification of dicotyledonous plants with a glycolate oxidizing pathway Genetics and Molecular Research 1.184 Bai XL. et al. 2011 Jul  http://www.ncbi.nlm.nih.gov/pubmed/21751162
Allopolyploid speciation and ongoing backcrossing between diploid progenitor and tetraploid progeny lineages in the Achillea millefolium species complex: analyses of single-copy nuclear genes and genomic AFLP BMC Evolutionary Biology 3.521 Ma JX. et al. 2010 Apr  http://www.ncbi.nlm.nih.gov/pubmed/20388203
A dsRNA-binding protein MdDRB1 associated with miRNA biogenesis modifies adventitious rooting and tree architecture in apple Plant Biotechnology Journal 6.279 Chun-Xiang You, et al. 2014 Feb

http://onlinelibrary.wiley.com/doi/10.1111/pbi.12125/abstract?deniedAccessCustomisedMessage
=&userIsAuthenticated=false

Detection and Identification of an Elm Yellows Group Phytoplasma Associated with Camellia in China Journal of Phytopathology 2.746 Ying Gao,et al. 2014 Dec http://onlinelibrary.wiley.com/doi/10.1111/jph.12354/abstract?deniedAccessCustomisedMessage=
&userIsAuthenticated=false
Long-term application of fresh and composted manure increase tetracycline resistance in the arable soil of eastern China Science of The Total Environment 4.099 Shuang Peng, et al.   2014 Nov http://www.sciencedirect.com/science/article/pii/S0048969714015927
Near Full-Length Identification of a Novel HIV-1 CRF01_AE/B/C Recombinant in Northern Myanmar AIDS Research and Human Retroviruses   Zhou Yan-Heng, et al.     2015 Jun http://online.liebertpub.com/doi/abs/10.1089/AID.2015.0021
Phytoplasma associated with Chinese tallow tree yellowing disease in China represents a new 16SrIII subgroup Forest Pathology 2.62 Y. Gao, et al. 2014 Jun http://onlinelibrary.wiley.com/doi/10.1111/efp.12128/abstract;jsessionid=
22924CB60930D262C9BA9E13DB263B98.f01t03?deniedAccessCustomisedMessage=&userIsAuthenticated=false

 

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