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  • Product Name: TransTaq® DNA Polymerase High Fidelity(HiFi)
  • Catalog Number: AP131
  • Date: 2015-10-25
  • Views: 466
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    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
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TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease. TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.



TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.



• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR


Unit Definition

One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

TransTaq® HiFi DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq® HiFi DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA


Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers


10×TransTaq® HiFi Buffer

200 mM Tris-HCl (pH 9.0), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 10% Glycerol, others



Contents& storage



Citations & references


Literature Journal IF Author Date Link
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi PLoS One 3.73 Wang B. et al. 2013
Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica Maxim Journal of Integrative Plant Biology 2.534 Li M, et al. 2013 Jun
The improved l-tryptophan production in recombinant Escherichia coli by expressing the polyhydroxybutyrate synthesis pathway Applied Microbiology and Biotechnology 3.689 Gu P, et al. 2013 May
Phylogenetic analysis using E2 gene of classical swine fever virus reveals a new subgenotype in China Infection, Genetics and Evolution 3.015 DL Jiang et al.       2013 July
A Modified TALEN-Based Strategy for Rapidly and Efficiently Generating Knockout Mice for Kidney Development Studies PloS one 3.73 Yunhong Liu,et al,      2014 Jan
New Amynthas species (Oligochaeta: Megascolecidae) from south and central Hainan Island, China and estimates of evolutionary divergence among some corticis-group species Journal of Natural History  0.881 Jing Suna et,al.      2013 Feb
Modulation of calcineurin activity in mouse brain by chronic oral administration of cyclosporine A IUBMB Life 2.789 Liu H, et al. 2013 May
One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli Microbial Cell Factories 3.552 Gu P. et al. 2012 Mar 
Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition Plant Physiology and Biochemistry 2.838 Xiang L. et al.  2012 Sep
Kernel lysine content does not increase in some maize opaque2 mutants Planta 3 Zhao G. et al.  2012 Jan
Nuclear and plastid haplotypes suggest rapid diploid and polyploid speciation in the N Hemisphere Achillea millefolium complex (Asteraceae) BMC Evolutionary Biology 3.521 Guo YP. et al.  2012 Jan
Plasmid construction for genetic modification of dicotyledonous plants with a glycolate oxidizing pathway Genetics and Molecular Research 1.184 Bai XL. et al. 2011 Jul
Allopolyploid speciation and ongoing backcrossing between diploid progenitor and tetraploid progeny lineages in the Achillea millefolium species complex: analyses of single-copy nuclear genes and genomic AFLP BMC Evolutionary Biology 3.521 Ma JX. et al. 2010 Apr
A dsRNA-binding protein MdDRB1 associated with miRNA biogenesis modifies adventitious rooting and tree architecture in apple Plant Biotechnology Journal 6.279 Chun-Xiang You, et al. 2014 Feb

Detection and Identification of an Elm Yellows Group Phytoplasma Associated with Camellia in China Journal of Phytopathology 2.746 Ying Gao,et al. 2014 Dec
Long-term application of fresh and composted manure increase tetracycline resistance in the arable soil of eastern China Science of The Total Environment 4.099 Shuang Peng, et al.   2014 Nov
Near Full-Length Identification of a Novel HIV-1 CRF01_AE/B/C Recombinant in Northern Myanmar AIDS Research and Human Retroviruses   Zhou Yan-Heng, et al.     2015 Jun
Phytoplasma associated with Chinese tallow tree yellowing disease in China represents a new 16SrIII subgroup Forest Pathology 2.62 Y. Gao, et al. 2014 Jun;jsessionid=


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