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  • Product Name: TransStart® FastPfu DNA Polymerase
  • Catalog Number: AP221 
  • Date: 2015-10-25
  • Views: 164
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    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
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TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.



• Extension rate is about 2-4 kb/min.
TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.



• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates


Unit Definition

One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

TransStart® FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol


TransStart® FastPfu Buffer with 20 mM MgSO4

100 mM Tris-SO4 (pH 9.2), 50 mM (NH4)2SO4, 200 mM KCl, 10 mM MgSO4, 10% Glycerol, others


Contents& storage

Citations & references


Literature Journal IF Author Date Link
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A Basic Helix-Loop-Helix Transcription Factor, PtrbHLH, of Poncirus trifoliata Confers Cold Tolerance and Modulates Peroxidase-Mediated Scavenging of Hydrogen Peroxide Plant Physiology 6.555 Huang X S, et al. 2013 Jun
Regio-and Stereospecific Prenylation of Flavonoids by Sophora flavescens Prenyltransferase Advanced Synthesis & Catalysis 5.535 Chen R, et al. 2013 Jun
Does self-construal predict activity in the social brain network? A genetic moderation effect Social Cognitive and Affective Neuroscience 5.042 Ma Y, et al. 2013 Sep
Analysis of oxygen reduction and microbial community of air-diffusion biocathode in microbial fuel cells Bioresource Technology 4.75 Wang Z, et al. 2013 Sep
Correlating microbial community structure and composition with aeration intensity in submerged membrane bioreactors by 454 high-throughput pyrosequencing Water Research 4.655 Ma J, et al. 2013 Feb
Stability and iron oxidation properties of a novel homopolymeric plant ferritin from adzuki bean seeds: A comparative analysis with recombinant soybean seed H-1 chain ferritin Biochimica et Biophysica Acta 3.848 Li M, et al. 2013 Apr
Novel Split-Luciferase-Based Genetically Encoded Biosensors for Noninvasive Visualization of Rho GTPases PLoS One 3.73 Leng W, et al. 2013 Apr
The Microbial Community in the Feces of the White Rhinoceros (Ceratotherium simum) as Determined by Barcoded Pyrosequencing Analysis PLoS One 3.73 Bian G,et al. 2013 Jul
Manure Refinement Affects Apple Rhizosphere Bacterial Community Structure: A Study in Sandy Soil PLoS One 3.73 Zhang Q, et al. 2013 Oct
Bacterial communities in manures of piglets and adult pigs bred with different feeds revealed by 16S rDNA 454 pyrosequencing Applied Microbiology and Biotechnology 3.689 Lu X M, et al. 2013 Sep
 Fungal networks in yield-invigorating and-debilitating soils induced by prolonged potato monoculture Soil Biology and Biochemistry 3.654 Lu LH, et al. 2013 Oct
GenR, an IclR-type regulator, activates and represses the transcription of gen genes involved in 3-hydroxybenzoate and gentisate catabolism in Corynebacterium glutamicum Journal of Bacteriology 3.825 Chao H. et al. 2013 Apr
Phylogenetic Diversity of Actinobacteria Associated with Soft Coral Alcyonium gracllimum and Stony Coral Tubastraea coccinea in the East China Sea Microbial Ecology 3.277 Yang S, et al. 2013 Jul
Analysis of the bacterial community in the two typical intertidal sediments of Bohai Bay, China by pyrosequencing Marine Pollution Bulletin 2.531 Wang L, et al. 2013 Jul
Microbial communities in an anaerobic dynamic membrane bioreactor (AnDMBR) for municipal wastewater treatment: Comparison of bulk sludge and cake layer Process Biochemistry 2.414 Ma J, et al. 2013 Mar
Fecal microbiota of piglets prefer utilizing DL-lactate mixture as compared to D-lactate and L-lactate in vitro Anaerobe 2.022 Su Y, et al. 2013 Feb
Cloning, expression and characterization of a phosphoglucomutase/phosphomannomutase from sphingan-producing Sphingomonas sanxanigenens Biotechnology Letters 1.853 Huang H, et al. 2013 Aug
Comprehensive analysis of the homeodomain-leucine zipper IV transcription factor family in Cucumis sativus Genome 1.668 Fu R, et al. 2013 Jul
Impact of subacute ruminal acidosis on the diversity of liquid and solid-associated bacteria in the rumen of goats World Journal of Microbiology and Biotechnology 1.532 Huo W, et al. 2013 Sep
Use of Pyrosequencing to Characterize the Microbiota in the Ileum of Goats Fed with Increasing Proportion of Dietary Grain Current Microbiology 1.52 Mao S, et al. 2013 Sep
Novel mutations of CYP27B1 gene lead to reduced activity of 1α-hydroxylase in Chinese patients Bone 4.023 Cui N. et al. 2012 Sep
The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis The Plant Jounal 6.16 Wang Y, et al. 2012 Jan
Genome-wide identification and characterization of R2R3MYB family in Cucumis sativus PLoS One 3.73 Li Q. et al. 2012
Bacterial and Archaeal symbionts in the south China Sea sponge Phakellia fusca: community structure, relative abundance, and ammonia-oxidizing populations Marine Biotechnology 2.739 Han M. et al. 2012 Dec
An Efficient Intergeneric Conjugation of DNA from Escherichia coli to Mycelia of the Lincomycin-Producer Streptomyces lincolnensis International Journal of Molecular Sciences 2.598 Du L, et al. 2012
The diversity of the fecal bacterial community and its relationship with the concentration of volatile fatty acids in the feces during subacute rumen acidosis in dairy cows BMC Veterinary Research 1.861 Mao S, et al. 2012 Dec
Population differentiation and phylogeographic pattern of a relict species, Conandron ramondioides (Gesneriaceae), revealed from sequence polymorphism and haplotypes of the CYCLOIDEA gene Journal of Systematics and Evolution 1.851 Xiao LH, et al. 2012 Jan
Shifts in microbial community function and structure along the successional gradient of coastal wetlands in Yellow River Estuary European Journal of Soil Biology 1.838 Yu Y, et al. 2012
Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO2 assimilation Acta biochimica et biophysica Sinica 1.807 Peng N, et al. 2012 Aug
Detection and quantification of cultured marine Alexandrium species by real-time PCR World Journal of Microbiology and Biotechnology 1.532 Zhang F, Li Z.  2012 Dec
Cryptic diversity in the pen shell Atrina pectinata (Bivalvia: Pinnidae): high divergence and hybridization revealed by molecular and morphological data Molecular Ecology 5.522 Liu J. et al.  2011 Oct
Furazolidone induced oxidative DNA damage via up-regulating ROS that caused cell cycle arrest in human hepatoma G2 cells Toxicology Letters 3.145 Jin X, et al. 2011 Mar
Comparison of expression, purification and characterization of a new pectate lyase from Phytophthora capsici using two different methods BMC Biotechnology 2.165 Wang H, et al. 2011 Apr
Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop Biotechnology Letters 1.853 Wei Z, et al. 2011 Dec
Dissection of the bridging pattern of bovicin HJ50, a lantibiotic containing a characteristic disulfide bridge Microbiological Research 1.993 Lin Y, et al. 2011 Mar
Development of a highly efficient gene targeting system allowing rapid genetic manipulations in Penicillium decumbens Applied Microbiology and Biotechnology 3.689 Li ZH. et al. 2010 Jul
Systematic metabolic engineering of Escherichia coli for high-yield production of fuel bio-chemical 2,3-butanediol Metabolic Engineering 8.258 Youqiang Xu, et al. 2014 May
Characterization of genotype IX Newcastle disease virus strains isolated from wild birds in the northern Qinling Mountains, China Virus Genes 1.769 Xuji Duan, et al. 2014 Feb
Glycerol Dehydrogenase Plays a Dual Role in Glycerol Metabolism and 2,3-Butanediol Formation in Klebsiella pneumoniae The Journal of Biological Chemistry 4.651 Yu Wang, et al. 2014 Feb
Integration and Expression of gfp in the Plastid of Medicago sativa L. Chloroplast Biotechnology   Shaochen Xing, et al. 2014
Characterization of pMC11, a plasmid with dual origins of replication isolated from Lactobacillus casei MCJ and construction of shuttle vectors with each replicon Applied Microbiology and Biotechnology 3.689 Zhengjun Chen, et al. 2014 Jul
Diversity, abundance, and spatial distribution of riverine microbial communities response to effluents from swine farm versus farmhouse restaurant Applied Microbiology and Biotechnology 3.689 Xiao-Ming Lu, et al. 2014 May
Antimycobacterial activity of Pichia pastoris-derived mature bovine neutrophil β-defensins 5 European Journal of Clinical Microbiology & Infectious Diseases 3.024 J. Kang, et al. 2014 May
A comparative study of microbial communities in soils amended by manure from pigs fed with organic versus synthetic feeds Toxicological and Environmental Chemistry 0.825 Xiao-Ming Lu, et al. 2014 Jun
Deletion of meso-2,3-butanediol dehydrogenase gene budC for enhanced D-2,3-butanediol production in Bacillus licheniformis Biotechnology for Biofuels 5.552 Gaofu Qi, et al. 2014
Effects of river sediment addition to the beach microbial communities Toxicological and Environmental Chemistry 0.825 Xiao-Ming Lu, et al. 2014 Apr
Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis Functional Plant Biology 2.471 Ronghua Xu, et al. 2013 Nov
Pyrosequencing technology reveals the impact of different manure doses on the bacterial community in apple rhizosphere soil Applied Soil Ecology 2.106 Jian Sun, et al. 2014 Jun
A novel low-temperature-active pectin methylesterase from Penicillium chrysogenum F46 with high efficiency in fruit firming Food Chemistry 3.334 Xia Pan, et al. 2014 Nov
GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress Plant Molecular Biology 4.15 Zhiyong Ni, et al. 2013 Mar
 Creation and validation of a widely applicable multiple gene transfer vector system for stable transformation in plant. Plant molecular biology 3.518 Quanxi Sun,et al. 2013 Jan
Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat Journal of Experimental Botany 5.364 Shanjun Tian,et al. 2013 Mar
Tetrapyrrole biosynthetic enzyme protoporphyrinogen
IX oxidase 1 is required for plastid RNA editing
PNAS 10.727 Fan Zhang, et al. 2013 Dec
The heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5 controls Caenorhabditis elegans Q neuroblast polarization during migration Developmental biology 3.637 Xing Liang, et al. 2015 Mar
Sarcomeres Pattern Proprioceptive Sensory Dendritic Endings through UNC-52/Perlecan in C. elegans Developmental cell 10.366 Xiangming Wang, et al.   2015 May
Diversity analysis of the bacterial community in tobacco waste extract during reconstituted tobacco process Applied Microbiology and Biotechnology 3.337 Huagui Liu, et al.    2015 Jan
Parental material and cultivation determine soil bacterial community structure and fertility FEMS Microbiology Ecology 3.568 L Sun, et al.       2014 Dec
Short-term changes in total heavy metal concentration and bacterial community composition after replicated and heavy application of pig manure-based compost in an organic vegetable production system Biology and Fertility of Soils 3.398 Wei Tian, et al. 2015 Mar
Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P1 Food Chemistry 3.391 Xia Pan, et al. 2015 May
Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. Strigosa Journal of Applied Genetics 1.477 Lumin Qin, et al. 2015 Feb
Enhanced Heavy Metal Tolerance and Accumulation by Transgenic Sugar Beets Expressing Streptococcus thermophilus StGCS-GS in the Presence of Cd, Zn and Cu Alone or in Combination PLoS One 3.23 Dali Liu, et al.  2015 Jun
Mechanisms of soil acidification reducing bacterial diversity Soil Biology and Biochemistry 3.932 Ximei Zhang, et al. 2014 Nov
CO2 sequestration by methanogens in activated sludge for methane production Applied Energy 5.613 Nazlina Haiza Mohd Yasin, et al.    2015 Jan
Denitrifying sulfide removal process on high-salinity wastewaters Applied Microbiology and Biotechnology 3.337 Chunshuang Liu, et al.    2015 Mar
Use of a Novel Integrase-Deficient Lentivirus for Targeted Anti-Cancer Therapy With Survivin Promoter-Driven Diphtheria Toxin A Medicine 4.723 Baoshun Lin, et al.   2015 Sep
Improving the Thermostability and Activity of a Thermophilic Subtilase by Incorporating Structural Elements of Its Psychrophilic Counterpart Appl. Environ. Microbiol.   Bi-Lin Xu, et al.  2015 Jun
Restoration of Bioactive Lantibiotic Suicin from a Remnant lan Locus of Pathogenic Streptococcus suis Serotype 2 applied and environmental microbiology 3.678 Jiang Wang et al. 2014 Jun


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