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  • Product Name: TransStart FastPfu DNA Polymerase
  • Catalog Number: AP221 
  • Date: 2015-10-25
  • Views: 43
  • Sold amount:0Piece
    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
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Overview

Description

TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

 

Highlights

• Extension rate is about 2-4 kb/min.
TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

 

Applications

• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

 

Unit Definition

One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74oC.

 

Quality Control

TransStart® FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

 

Storage Buffer

50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol

 

TransStart® FastPfu Buffer with 20 mM MgSO4

100 mM Tris-SO4 (pH 9.2), 50 mM (NH4)2SO4, 200 mM KCl, 10 mM MgSO4, 10% Glycerol, others

 

Contents& storage

Citations & references

 

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A Basic Helix-Loop-Helix Transcription Factor, PtrbHLH, of Poncirus trifoliata Confers Cold Tolerance and Modulates Peroxidase-Mediated Scavenging of Hydrogen Peroxide Plant Physiology 6.555 Huang X S, et al. 2013 Jun http://www.ncbi.nlm.nih.gov/pubmed/23624854
Regio-and Stereospecific Prenylation of Flavonoids by Sophora flavescens Prenyltransferase Advanced Synthesis & Catalysis 5.535 Chen R, et al. 2013 Jun http://onlinelibrary.wiley.com/doi/10.1002/adsc.201300196/abstract?deniedAccessCustomisedMessage=&userIsAuthenticated=false
Does self-construal predict activity in the social brain network? A genetic moderation effect Social Cognitive and Affective Neuroscience 5.042 Ma Y, et al. 2013 Sep  http://www.ncbi.nlm.nih.gov/pubmed/24009354
Analysis of oxygen reduction and microbial community of air-diffusion biocathode in microbial fuel cells Bioresource Technology 4.75 Wang Z, et al. 2013 Sep  http://www.ncbi.nlm.nih.gov/pubmed/23859984
Correlating microbial community structure and composition with aeration intensity in submerged membrane bioreactors by 454 high-throughput pyrosequencing Water Research 4.655 Ma J, et al. 2013 Feb http://www.ncbi.nlm.nih.gov/pubmed/23200801
Stability and iron oxidation properties of a novel homopolymeric plant ferritin from adzuki bean seeds: A comparative analysis with recombinant soybean seed H-1 chain ferritin Biochimica et Biophysica Acta 3.848 Li M, et al. 2013 Apr http://www.ncbi.nlm.nih.gov/pubmed/23313843
Novel Split-Luciferase-Based Genetically Encoded Biosensors for Noninvasive Visualization of Rho GTPases PLoS One 3.73 Leng W, et al. 2013 Apr http://www.ncbi.nlm.nih.gov/pubmed/23614039
The Microbial Community in the Feces of the White Rhinoceros (Ceratotherium simum) as Determined by Barcoded Pyrosequencing Analysis PLoS One 3.73 Bian G,et al. 2013 Jul  http://www.ncbi.nlm.nih.gov/pubmed/23922920
Manure Refinement Affects Apple Rhizosphere Bacterial Community Structure: A Study in Sandy Soil PLoS One 3.73 Zhang Q, et al. 2013 Oct http://www.ncbi.nlm.nih.gov/pubmed/24155909
Bacterial communities in manures of piglets and adult pigs bred with different feeds revealed by 16S rDNA 454 pyrosequencing Applied Microbiology and Biotechnology 3.689 Lu X M, et al. 2013 Sep  http://www.ncbi.nlm.nih.gov/pubmed/24068333
 Fungal networks in yield-invigorating and-debilitating soils induced by prolonged potato monoculture Soil Biology and Biochemistry 3.654 Lu LH, et al. 2013 Oct http://www.sciencedirect.com/science/article/pii/S0038071713001971
GenR, an IclR-type regulator, activates and represses the transcription of gen genes involved in 3-hydroxybenzoate and gentisate catabolism in Corynebacterium glutamicum Journal of Bacteriology 3.825 Chao H. et al. 2013 Apr http://www.ncbi.nlm.nih.gov/pubmed/23354754
Phylogenetic Diversity of Actinobacteria Associated with Soft Coral Alcyonium gracllimum and Stony Coral Tubastraea coccinea in the East China Sea Microbial Ecology 3.277 Yang S, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23503990
Analysis of the bacterial community in the two typical intertidal sediments of Bohai Bay, China by pyrosequencing Marine Pollution Bulletin 2.531 Wang L, et al. 2013 Jul  http://www.ncbi.nlm.nih.gov/pubmed/23660440
Microbial communities in an anaerobic dynamic membrane bioreactor (AnDMBR) for municipal wastewater treatment: Comparison of bulk sludge and cake layer Process Biochemistry 2.414 Ma J, et al. 2013 Mar http://www.sciencedirect.com/science/article/pii/S1359511313000421
Fecal microbiota of piglets prefer utilizing DL-lactate mixture as compared to D-lactate and L-lactate in vitro Anaerobe 2.022 Su Y, et al. 2013 Feb http://www.ncbi.nlm.nih.gov/pubmed/23201433
Cloning, expression and characterization of a phosphoglucomutase/phosphomannomutase from sphingan-producing Sphingomonas sanxanigenens Biotechnology Letters 1.853 Huang H, et al. 2013 Aug http://www.ncbi.nlm.nih.gov/pubmed/23546942
Comprehensive analysis of the homeodomain-leucine zipper IV transcription factor family in Cucumis sativus Genome 1.668 Fu R, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/24099392
Impact of subacute ruminal acidosis on the diversity of liquid and solid-associated bacteria in the rumen of goats World Journal of Microbiology and Biotechnology 1.532 Huo W, et al. 2013 Sep http://www.ncbi.nlm.nih.gov/pubmed/24068532
Use of Pyrosequencing to Characterize the Microbiota in the Ileum of Goats Fed with Increasing Proportion of Dietary Grain Current Microbiology 1.52 Mao S, et al. 2013 Sep http://www.ncbi.nlm.nih.gov/pubmed/23636494
Novel mutations of CYP27B1 gene lead to reduced activity of 1α-hydroxylase in Chinese patients Bone 4.023 Cui N. et al. 2012 Sep http://www.ncbi.nlm.nih.gov/pubmed/22588163
The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis The Plant Jounal 6.16 Wang Y, et al. 2012 Jan http://www.ncbi.nlm.nih.gov/pubmed/21910774
Genome-wide identification and characterization of R2R3MYB family in Cucumis sativus PLoS One 3.73 Li Q. et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/23110079
Bacterial and Archaeal symbionts in the south China Sea sponge Phakellia fusca: community structure, relative abundance, and ammonia-oxidizing populations Marine Biotechnology 2.739 Han M. et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22310803
An Efficient Intergeneric Conjugation of DNA from Escherichia coli to Mycelia of the Lincomycin-Producer Streptomyces lincolnensis International Journal of Molecular Sciences 2.598 Du L, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/22606009
The diversity of the fecal bacterial community and its relationship with the concentration of volatile fatty acids in the feces during subacute rumen acidosis in dairy cows BMC Veterinary Research 1.861 Mao S, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/23217205
Population differentiation and phylogeographic pattern of a relict species, Conandron ramondioides (Gesneriaceae), revealed from sequence polymorphism and haplotypes of the CYCLOIDEA gene Journal of Systematics and Evolution 1.851 Xiao LH, et al. 2012 Jan http://onlinelibrary.wiley.com/doi/10.1111/j.17596831.2011.00166.x
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Shifts in microbial community function and structure along the successional gradient of coastal wetlands in Yellow River Estuary European Journal of Soil Biology 1.838 Yu Y, et al. 2012 http://www.sciencedirect.com/science/article/pii/S1164556311000860
Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO2 assimilation Acta biochimica et biophysica Sinica 1.807 Peng N, et al. 2012 Aug http://www.ncbi.nlm.nih.gov/pubmed/22710261
Detection and quantification of cultured marine Alexandrium species by real-time PCR World Journal of Microbiology and Biotechnology 1.532 Zhang F, Li Z.  2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22864601
Cryptic diversity in the pen shell Atrina pectinata (Bivalvia: Pinnidae): high divergence and hybridization revealed by molecular and morphological data Molecular Ecology 5.522 Liu J. et al.  2011 Oct http://www.ncbi.nlm.nih.gov/pubmed/21923775
Furazolidone induced oxidative DNA damage via up-regulating ROS that caused cell cycle arrest in human hepatoma G2 cells Toxicology Letters 3.145 Jin X, et al. 2011 Mar http://www.ncbi.nlm.nih.gov/pubmed/21195149
Comparison of expression, purification and characterization of a new pectate lyase from Phytophthora capsici using two different methods BMC Biotechnology 2.165 Wang H, et al. 2011 Apr  http://www.ncbi.nlm.nih.gov/pubmed/21470403
Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop Biotechnology Letters 1.853 Wei Z, et al. 2011 Dec http://www.ncbi.nlm.nih.gov/pubmed/21785988
Dissection of the bridging pattern of bovicin HJ50, a lantibiotic containing a characteristic disulfide bridge Microbiological Research 1.993 Lin Y, et al. 2011 Mar http://www.ncbi.nlm.nih.gov/pubmed/20630724
Development of a highly efficient gene targeting system allowing rapid genetic manipulations in Penicillium decumbens Applied Microbiology and Biotechnology 3.689 Li ZH. et al. 2010 Jul http://www.ncbi.nlm.nih.gov/pubmed/20393703
Systematic metabolic engineering of Escherichia coli for high-yield production of fuel bio-chemical 2,3-butanediol Metabolic Engineering 8.258 Youqiang Xu, et al. 2014 May http://www.sciencedirect.com/science/article/pii/S109671761400010X
Characterization of genotype IX Newcastle disease virus strains isolated from wild birds in the northern Qinling Mountains, China Virus Genes 1.769 Xuji Duan, et al. 2014 Feb http://link.springer.com/article/10.1007/s11262-013-0987-y
Glycerol Dehydrogenase Plays a Dual Role in Glycerol Metabolism and 2,3-Butanediol Formation in Klebsiella pneumoniae The Journal of Biological Chemistry 4.651 Yu Wang, et al. 2014 Feb http://www.jbc.org/content/289/9/6080.short
Integration and Expression of gfp in the Plastid of Medicago sativa L. Chloroplast Biotechnology   Shaochen Xing, et al. 2014 http://link.springer.com/protocol/10.1007/978-1-62703-995-6_25
Characterization of pMC11, a plasmid with dual origins of replication isolated from Lactobacillus casei MCJ and construction of shuttle vectors with each replicon Applied Microbiology and Biotechnology 3.689 Zhengjun Chen, et al. 2014 Jul http://link.springer.com/article/10.1007/s00253-014-5649-z
Diversity, abundance, and spatial distribution of riverine microbial communities response to effluents from swine farm versus farmhouse restaurant Applied Microbiology and Biotechnology 3.689 Xiao-Ming Lu, et al. 2014 May http://link.springer.com/article/10.1007/s00253-014-5772-x
Antimycobacterial activity of Pichia pastoris-derived mature bovine neutrophil β-defensins 5 European Journal of Clinical Microbiology & Infectious Diseases 3.024 J. Kang, et al. 2014 May http://link.springer.com/article/10.1007/s10096-014-2152-5
A comparative study of microbial communities in soils amended by manure from pigs fed with organic versus synthetic feeds Toxicological and Environmental Chemistry 0.825 Xiao-Ming Lu, et al. 2014 Jun http://www.tandfonline.com/doi/abs/10.1080/02772248.2014.939981#.U7yeqtPlqM8
Deletion of meso-2,3-butanediol dehydrogenase gene budC for enhanced D-2,3-butanediol production in Bacillus licheniformis Biotechnology for Biofuels 5.552 Gaofu Qi, et al. 2014 http://www.biomedcentral.com/content/pdf/1754-6834-7-16.pdf
Effects of river sediment addition to the beach microbial communities Toxicological and Environmental Chemistry 0.825 Xiao-Ming Lu, et al. 2014 Apr http://www.tandfonline.com/doi/abs/10.1080/02772248.2014.907410#.U7zlUtPlqM8
Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis Functional Plant Biology 2.471 Ronghua Xu, et al. 2013 Nov http://www.publish.csiro.au/?paper=FP12388
Pyrosequencing technology reveals the impact of different manure doses on the bacterial community in apple rhizosphere soil Applied Soil Ecology 2.106 Jian Sun, et al. 2014 Jun http://www.sciencedirect.com/science/article/pii/S0929139314000559
A novel low-temperature-active pectin methylesterase from Penicillium chrysogenum F46 with high efficiency in fruit firming Food Chemistry 3.334 Xia Pan, et al. 2014 Nov http://www.sciencedirect.com/science/article/pii/S0308814614006268
GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress Plant Molecular Biology 4.15 Zhiyong Ni, et al. 2013 Mar http://link.springer.com/article/10.1007/s11103-013-0040-5
 Creation and validation of a widely applicable multiple gene transfer vector system for stable transformation in plant. Plant molecular biology 3.518 Quanxi Sun,et al. 2013 Jan http://link.springer.com/article/10.1007/s11103-013-0096-2
Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat Journal of Experimental Botany 5.364 Shanjun Tian,et al. 2013 Mar http://jxb.oxfordjournals.org/content/64/7/2063.short
Tetrapyrrole biosynthetic enzyme protoporphyrinogen
IX oxidase 1 is required for plastid RNA editing
PNAS 10.727 Fan Zhang, et al. 2013 Dec http://www.pnas.org/content/111/5/2023.short
The heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5 controls Caenorhabditis elegans Q neuroblast polarization during migration Developmental biology 3.637 Xing Liang, et al. 2015 Mar http://www.sciencedirect.com/science/article/pii/S0012160615000159
Sarcomeres Pattern Proprioceptive Sensory Dendritic Endings through UNC-52/Perlecan in C. elegans Developmental cell 10.366 Xiangming Wang, et al.   2015 May http://www.cell.com/developmental-cell/abstract/S1534-5807(15)00178-1?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1534580715001781%3Fshowall%3Dtrue
Diversity analysis of the bacterial community in tobacco waste extract during reconstituted tobacco process Applied Microbiology and Biotechnology 3.337 Huagui Liu, et al.    2015 Jan http://link.springer.com/article/10.1007/s00253-014-5960-8
Parental material and cultivation determine soil bacterial community structure and fertility FEMS Microbiology Ecology 3.568 L Sun, et al.       2014 Dec http://femsec.oxfordjournals.org/content/91/1/1.7.abstract
Short-term changes in total heavy metal concentration and bacterial community composition after replicated and heavy application of pig manure-based compost in an organic vegetable production system Biology and Fertility of Soils 3.398 Wei Tian, et al. 2015 Mar http://link.springer.com/article/10.1007/s00374-015-1005-4
Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P1 Food Chemistry 3.391 Xia Pan, et al. 2015 May http://www.sciencedirect.com/science/article/pii/S030881461500744X
Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. Strigosa Journal of Applied Genetics 1.477 Lumin Qin, et al. 2015 Feb http://link.springer.com/article/10.1007/s13353-014-0229-6
Enhanced Heavy Metal Tolerance and Accumulation by Transgenic Sugar Beets Expressing Streptococcus thermophilus StGCS-GS in the Presence of Cd, Zn and Cu Alone or in Combination PLoS One 3.23 Dali Liu, et al.  2015 Jun http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0128824
Mechanisms of soil acidification reducing bacterial diversity Soil Biology and Biochemistry 3.932 Ximei Zhang, et al. 2014 Nov http://www.sciencedirect.com/science/article/pii/S0038071714003824
CO2 sequestration by methanogens in activated sludge for methane production Applied Energy 5.613 Nazlina Haiza Mohd Yasin, et al.    2015 Jan http://www.sciencedirect.com/science/article/pii/S0306261914013269
Denitrifying sulfide removal process on high-salinity wastewaters Applied Microbiology and Biotechnology 3.337 Chunshuang Liu, et al.    2015 Mar http://link.springer.com/article/10.1007/s00253-015-6505-5
Use of a Novel Integrase-Deficient Lentivirus for Targeted Anti-Cancer Therapy With Survivin Promoter-Driven Diphtheria Toxin A Medicine 4.723 Baoshun Lin, et al.   2015 Sep http://journals.lww.com/md-journal/pages/articleviewer.aspx?year=2015&issue=08010&article=00043&type=abstract
Improving the Thermostability and Activity of a Thermophilic Subtilase by Incorporating Structural Elements of Its Psychrophilic Counterpart Appl. Environ. Microbiol.   Bi-Lin Xu, et al.  2015 Jun http://aem.asm.org/content/81/18/6302.short
Restoration of Bioactive Lantibiotic Suicin from a Remnant lan Locus of Pathogenic Streptococcus suis Serotype 2 applied and environmental microbiology 3.678 Jiang Wang et al. 2014 Jun http://aem.asm.org/content/80/3/1062.short

 

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