TransDetect^® Fluorescence RNase Detection Kit uses fluorescence-based method to rapidly and highly sensitively detect residual RNase in samples. Based on the principle of FRET: RNA probe tagged with a fluorescent reporter at the 5' end and a quencher at the 3' end is used as substrate. In the absence of RNase in sample, the quencher and reporter are near one another, and no fluorescent signal is generated; When RNase is present, the quencher and reporter are spatially separated in solution as RNA substrate is cleaved. This causes a fluorescence signal that can be detected. The RNA probe substrate has been optimized, which can detect as low as 0.3 pg of RNase A and has good detection effect on various RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease and Benzonase.

Applications

Suitable for detection of RNase in biological materials, experimental environments, reaction buffers, storage buffers, etc.

Storage

at -20℃ for two years

Shipping

Dry ice (-70℃)