pEASY®-Basic Seamless Cloning and Assembly Kit
Catalog Number： CU201-02
Price：Please inquire first
Product Details
This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments.
• Fast: 5~15 minutes.
• Simple: No restriction enzyme digestions.
• High efficiency: > 95% cloning efficiency.
• Seamless: No extra sequences introduced.
Single or multi fragments cloning and assembly.
at -20℃ for two years.
Dry ice (-70℃)
Component | CU201-02 | CU201-03 | CU201-13 |
2×Basic Assembly Mix | 100 μl | 3×100 μl | - |
2×Basic Assembly Mix (+Dye) | - | - | 3×100 μl |
Linearized pUC19 Control Vector (10 ng/μl) | 6 μl | 18 μl | 18 µl |
Control Insert (1 kb，20 ng/μl） | 6 μl | 18 μl | 18 µl |
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