pEASY®-Blunt Cloning Kit
Catalog Number: CB101-01
Price:Please inquire first
Product Details
pEASY®-Blunt Cloning Vector containing the LacZ gene allows for blue-white screening on plates supplemented with IPTG and X-gal. It is suitable for blunt-end cloning.
Features
• Fast: 5 minutes.
• Simple: Just add the fragment.
• High cloning efficiency: High positive cloning rate.
• Kanamycin and Ampicillin resistance genes for selection.
• M13 Forward Primer, M13 Reverse Primer and T7 Promoter Primer for sequencing.
• T7 promoter for in vitro transcription.
• Trans1-T1 competent cells have high transformation efficiency and rapid growth rate, ensuring the number of clones and saving screening time.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70℃ or below for six months; others at -18℃ or below for nine months
Shipping
Dry ice (-70℃)
Component | CB101-01 (20 rxns) | CB101-02 (60 rxns) |
pEASY ®-Blunt Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
Control Template (5 ng/μl) | 5 μl | 5 μl |
Control Primers (10 μM) | 5 μl | 5 μl |
M13 Forward Primer (10 μM) | 50 μl | 150 μl |
M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
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