Universal Nuclease (Benzonase^®) is a non-specific, broad-spectrum endonuclease derived from Serratia marcescens. It is produced through genetic engineering, expressed in Escherichia coli, and subsequently purified. Also known as a non-restriction endonuclease or broad-spectrum nuclease, this enzyme cleaves DNA and RNA strands indiscriminately at any nucleotide site, degrading nucleic acids into 5'-monophosphorylated oligonucleotides 2-5 bases in length. Consequently, Universal Nuclease efficiently degrades all forms of DNA and RNA (double-stranded, single-stranded, circular, or linear) without causing protein hydrolysis. It is widely used to remove nucleic acid residues and contaminants from biological products.

This kit employs a sandwich ELISA (Enzyme-Linked Immunosorbent Assay) method for the quantitative determination of Universal Nuclease residues in biological products. The microplate is pre-coated with a high-affinity Universal Nuclease capture antibody. Both standards/samples and a biotin-labeled Universal Nuclease detection antibody are added simultaneously to the wells. During incubation, any Universal Nuclease present in the sample binds specifically to both the immobilized capture antibody on the plate and the biotinylated detection antibody. After a wash step to remove unbound substances, Streptavidin conjugated to Horseradish Peroxidase (Streptavidin-HRP) is added and incubated. The high-affinity, non-covalent interaction between biotin on the detection antibody and streptavidin forms an "capture antibody - Universal Nuclease - detection antibody - Streptavidin-HRP" immune complex. Following another wash, the chromogenic substrate TMB is added to the wells. The HRP enzyme catalyzes the conversion of TMB into a blue product. The intensity of this colorimetric reaction is directly proportional to the concentration of Universal Nuclease in the sample. A stop solution is then added to terminate the reaction, and the absorbance is measured at 450 nm (with a reference wavelength of 570-630 nm). By constructing a standard curve from the absorbance values of the standards, the concentration of Universal Nuclease in the samples can be determined. This kit offers high specificity, superior sensitivity, and a more convenient operational procedure.

1 year in the dark at 2-8°C

Ice bag (4℃)